Distribution characteristic of MTA 1 and its relationship with MMP-9,VEGF-C protein expression in human laryngocarcinoma / 重庆医学

Objective To investigate the expression characteristic of metastasis associated 1(MTA1) protein in laryngocarcino-ma tissue and to analyze its relationship with matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor C(VEGF-C) proteinin .Methods The Western blot method was adopt to detect the expression levels of MTA1 ,MMP-9 and VEGF-C protein in the specimens of normal laryngeal mucosal tissue ,paracarcinoma tissue and tumor tissue from 40 cases of laryngocarcinoma .Re-sults Compared with the groups of laryngocarcinoma without lymph node metastasis ,paracarcinoma and normal tissue ,the expres-sion level of MTA1 protein in the laryngocarcinoma with lymph node metastasis group was significantly increased (P<0 .05) .The expression of MTA1 protein in the laryngocarcinoma tissue was significantly correlated with the lymph nodes metastasis ,tumor staging and differentiation degree of laryngocarcinoma (P<0 .05) and was positively correlated with the expression of VEGF-C and MMP-9 (P<0 .05) .Conclusion MTA1 may participate in the development ,progress and metastasis of laryngocarcinoma .Moreo-ver the regulating effect may exist among MTA1 ,MMP-9 and VEGF-C .

Expression and location of YB-1 protein in hepatocellular carcinoma and their clinical significance / 中华肝胆外科杂志

ObjectiveTo study the expression levels of YB-1 protein in hepatocellular carcinoma (HCC) tissues,tissues adjacent to tumour and normal liver tissues,and to explore the clinical significance.MethodsTo study the expression levels of YB-1 protein,immunochemistry was carried out on 58 HCC tissues and their corresponding adjacent tissues to tumour and on 20 normal liver tissues.The YB-1 protein with nuclear translocation was detected by Western blot in HCC tissues,tissues adjacent to tumour,normal liver tissues,HCC cell lines including QGY-7701 and SMMC-7721,and a normal hepatic cell line LO2.ResultsPositive signals of YB-1 protein were detected at a high level in HCC tissues (72.4％,42/58) when compared to tissues adjacent to tumour (41.4％,24/58) and to normal liver tissues (35 ％,7/20) (P＞0.05).The nuclear expression of YB-1 (31％,18/58) was significantly correlated with the pathologicalgrade,tumour size and portal venous invasion (P＜0.05).Using Western blot,the YB-1 protein with nuclear translocation was expressed at a higher level in HCC tissues (0.474±0.107) than in tissues adjacent to tumour and in normal liver tissues (P＜0.05).Similar results were obtained in HCC cell lines and the normal hepatic cell line (P＜0.05).ConclusionThe high expression of YB-1 in HCC and its nuclear translocation may be involved in human HCC progression.YB-1 may be a potential target for HCC treatment in the future.

Clinical significance of MRP8 and cypA expression in laryngeal squamous cell carcinoma tissues / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study differential expression of MRP8, CypA protein in the patients of laryngeal squamous cell carcinoma (LSCC) and the relationship in the development of LSCC.@*METHOD@#Immunohistochemistry was used to detect the expression of MRP8,CypA protein in LSCC tissues of 41 cases and matched paraneoplastic normal tissues of 41 cases,with results compared to the clinical data to determine significance.@*RESULT@#The expression of MRP8, CypA protein in carcinoma and normal tissues and composition of different positive grades were in statistical significance (P 0.05), but related to pathological stage (P < 0.05).@*CONCLUSION@#MRP8 protein is on intimate terms with different pathological differentiation stage of LSCC. MRP8, CypA protein may play an important role in the development and progression of LSCC.

Expression of vascular endothelial growth factor D (VEGF-D) and urokinase-type plasminogen activator in human rectal carcinoma and their relationship with tumor invasion and metastasis / 中华内分泌外科杂志

Objective To explore the expression of endothelial growth factor-D (VEGF-D) and uroki-nase-type plasminogen activator(uPA) in rectal carcinoma and to reveal their correlation to the tumor invasion and metastasis.Methods The expression of VEGF-D and uPA in 30 cases with rectal carcinoma and normal tissue was detected by immunohistochemistry and Western blot.Results The expression of VEGF-D and uPA was de-tected both in tumor tissue and normal tissue, but significantly higher in tumor tissue (P < 0.01), they expressed mostly in endochylema.The expression of VEGF-D or uPA was significantly higher in Dukes' stage C + D than that in Dukes' stage A + B(P <0.01), was also higher in tissues with lymph node metastasis or distant metasta-sis than those without metastasis(P <0.05 ,P <0.01).They were found to be lower in cancer tissue along with the differentiation degree increase(P < 0.05) .Condnsions The overexpression of VEGF-D and uPA in rectal carcinoma may play an important part in the tumorigenesis and progression of rectal carcinoma.

Relationship of VEGF-D and uPA with invasion and metastasis of human colon carcinoma / 第三军医大学学报

Objective To explore the expressions of endothelial growth factor-D (VEGF-D) and urokinase-type plasminogen activator (uPA) in colon carcinoma and to reveal their correlation with the tumor invasion and metastasis. Methods The expressions of VEGF-D and uPA were detected by immunohistochemistry and Western blotting in 30 specimens of colon carcinoma and 30 specimens of normal neighbouring colon tissue 5 cm away from the lesions. Results The expressions of VEGF-D and uPA mostly located in endochylema, less in nucleus, of both tumor tissue and normal tissue, and they were significantly higher in tumor tissue than normal tissue (P

Significance and expression of nuclear factor-?B in human breast cancer / 中国普通外科杂志

Objective To explore the expression and activation of nuclear transcription factor ?B(NF ?B) in human breast cancer (BC), and the relationship between the activity of NF ?B and malignant potential of BC. Methods The protein expression of NF ?B p65 in BC tissue and paratumor tissue were measured by Western blot; NF ?B DNA binding activity was examined by electrophoretic motility shift assay(EMSA)in 26 BC tissues and 12 paratumor tissues of BC.Results The levels of NF ?B p65 protein and NF ?B DNA binding activity in the tumor tissue were higher than those in the paratumor tissues(P